Anglin took advantage of a Danielle Oswald assist to score the game-winner. The Golden Eagles (14-2) scored first 19:06 into the match when Jessa Murray scored on an assist from Sara Fassbinder.
Pleasant Valley tied the score with 25 minutes remaining in regulation when Jesse Pryor connected on a Mindi Mappin assist.
Dubuque Wahlert advances to the state semfinals for the third time in four years. The Golden Eagles will face Iowa City High or Bettendorf at 9 a.m. Saturday.
Pleasant Valley ends its season at 10-7.
DUBUQUE WAHLERT 2, PLEASANT VALLEY 1
Dubuque Wahlert 1 1 -2
Pleasant Valley 0 1 -1
Scoring
DW - Jessa Murray (Sara Fassbinder), 19:06
PV - Jesse Pryor (Mindi Mappin), 55:00
DW - Kristin Anglin (Danielle Oswald), 74:10