2021 State Qualifying Time Standard: :59.53
| 1 |  Scarlet Martin, SRIowa City West | 54.90 |
| 2 |  Mikayla Kloth, SRAmes | 56.04 |
| 3 |  Grace Frericks, SRCedar Falls | 56.70 |
| 4 |  Olivia Swalley, JRJohnston | 57.61 |
| 5 | Ella Hochstetler, SR Iowa City West | 58.14 |
| 6 | Jordon Kloth, SR Ames | 58.37 |
| 7 |  Cassie Rounds, SRDowling Catholic | 58.41 |
| 8 |  Nora Kemp, SOWaukee | 58.59 |
| 9 | Mallory Kell, JR Waukee | 58.66 |
| 10 | Julia Sweetman, JR Dowling Catholic | 58.86 |
| 11 | Kendall Mallaro, SR Cedar Falls | 59.17 |
| 12 | Willa Hage, FR Waukee | 59.25 |
| 13 |  Hannah Nedder, SRNewton | 59.29 |
| 14 |  Adeline Li, SOValley | 59.32 |
| 15 |  Grace Hoeper, FRIowa City High | 59.46 |