| 1 | Hayley Kimmel FR | Linn-Mar | 1:05.09 | Linn-Mar Aquatic Center | 09/14/2019 |
| 2 | Mary Martin JR | Dowling Catholic | 1:05.70 | Dowling Catholic High School | 10/15/2019 |
| 3 | Olivia Masterson SR | Iowa City High | 1:05.73 | Mercer Park Aquatic Center and Scanlon Gym | 09/10/2019 |
| 4 | Meghan Donald SO | Ames | 1:06.68 | Dowling Catholic High School | 10/15/2019 |
| 5 | Arianna Ottavianelli SR | Bettendorf | 1:07.39 | Linn-Mar Aquatic Center | 09/14/2019 |
| 6 | Olivia Swalley FR | Johnston | 1:07.41 | Summit Middle School | 10/08/2019 |
| 7 | Emma Bachelder SR | Decorah | 1:07.52 | Mercer Park Aquatic Center and Scanlon Gym | 09/21/2019 |
| 8 | Taylor Buhr SO | Pleasant Valley | 1:07.75 | 09/24/2019 |
| 9 | Sami Roemer SR | Bettendorf | 1:08.36 | Clinton High School | 10/15/2019 |
| 10 | Lily Wright SR | Sioux City North | 1:08.47 | Valley High School | 09/28/2019 |
| 11 | Scarlet Martin SO | Iowa City West | 1:08.61 | Linn-Mar High School | 10/26/2019 |