100 Backstroke Time Standard: 1:00.09
| 1 |  Scarlet Martin, JRIowa City West | 56.57 |
| 2 |  Grace Frericks, JRCedar Falls | 57.51 |
| 3 |  Jessi Wigham, SOWaukee | 58.04 |
| 4 | Aurora Roghair, SR Iowa City West | 58.07 |
| 5 |  Annie Galvin, SRAmes | 58.08 |
| 6 |  Ellie Clark, SRDowling Catholic | 58.43 |
| 7 | Julia Sweetman, SO Dowling Catholic | 58.90 |
| 8 |  Hope Cvrk, SRSioux City North | 59.21 |
| 9 | Meghan Donald, JR Ames | 59.36 |
| 10 | Abby Wilcox, FR Dowling Catholic | 59.38 |
| 11 |  Alexis Henning, JRAnkeny | 59.47 |
| 12 | Carlee Wilkins, FR Iowa City West | 59.57 |
| 13 |  Hannah Cousins, FRDavenport Central | 59.58 |
| 14 | Abby Hala, JR Ames | 59.69 |
| 15 |  Abby Rastetter, SRJohnston | 59.79 |
| 16 |  Liz Purtle, SRBoone | 59.88 |
| 17 | Abby McCorkle, SR Ames | 59.97 |
| 18 |  Sydney Jones, SRCedar Rapids Washington | 1:00.02 |
| 19 | Payden Rafferty, FR Waukee | 1:00.04 |
| 20 | Olivia Swalley, SO Johnston | 1:00.05 |