2021 State Qualifying Standard: 1:08.67
| 1 |  Olivia Swalley, JRJohnston | 1:04.28 |
| 2 |  Willa Hage, FRWaukee | 1:06.07 |
| 3 |  Andra Robertson, FRAmes | 1:06.76 |
| 4 |  Taylor Buhr, SRPleasant Valley | 1:07.34 |
| 5 |  Kylee Brown, JRLewis Central | 1:07.37 |
| 6 | Reese Winer, SO Ames | 1:07.46 |
| 7 |  Emma Foth, JRDowling Catholic | 1:08.06 |
| 8 |  Isabel Heller, SODavenport Central | 1:08.30 |
| 9 | Dylan Hogenson, SO Waukee | 1:08.31 |
| 10 |  Hayley Kimmel, JRLinn-Mar | 1:08.38 |
| 11 |  Brooke Moser, SRSpencer | 1:08.45 |
| 12 |  Anja Peck, SOAnkeny | 1:08.50 |