2021 State Qualifying Standard: 2:12.95
| 1 |  Olivia Swalley, JRJohnston | 2:04.75 |
| 2 |  Meghan Donald, SRAmes | 2:06.79 |
| 3 |  Mallory Kell, JRWaukee | 2:07.84 |
| 4 |  Scarlet Martin, SRIowa City West | 2:08.73 |
| 5 | Willa Hage, FR Waukee | 2:09.05 |
| 6 | Nora Kemp, SO Waukee | 2:09.37 |
| 7 | Taylor Judas, SO Waukee | 2:09.45 |
| 8 |  Julia Sweetman, JRDowling Catholic | 2:09.72 |
| 9 | Zoe Davey, FR Waukee | 2:10.10 |
| 10 | Payden Rafferty, SO Waukee | 2:11.06 |
| 11 | Olivia Taeger, SR Iowa City West | 2:11.08 |
| 12 | Makala Hajek, JR Iowa City West | 2:11.28 |
| 13 |  Taylor Buhr, SRPleasant Valley | 2:12.13 |
| 14 |  Jenna Currier, SRCarroll | 2:12.38 |
| 15 |  Hayley Kimmel, JRLinn-Mar | 2:12.56 |
| 16 |  Grace Frericks, SRCedar Falls | 2:12.68 |
| 17 | Jade Roghair, JR Iowa City West | 2:12.70 |
| 18 | Andra Robertson, FR Ames | 2:12.76 |
| 19 | Abby Hala, SR Ames | 2:12.84 |
| 20 | Dylan Hogenson, SO Waukee | 2:12.93 |