2021 State Qualifying Time Standard: :24.79
| 1 |  Meghan Donald, SRAmes | 23.24 |
| 2 |  Scarlet Martin, SRIowa City West | 23.80 |
| 3 |  Grace Frericks, SRCedar Falls | 24.02 |
| 4 | Ella Hochstetler, SR Iowa City West | 24.28 |
| 5 |  Olivia Swalley, JRJohnston | 24.39 |
| 6 | Mikayla Kloth, SR Ames | 24.41 |
| 7 |  Hannah Cousins, SODavenport Central | R 24.49 |
| 8 |  Grace Hoeper, FRIowa City High | 24.51 |
| 9 |  Payden Rafferty, SOWaukee | 24.53 |
| 10 | Sophie McCorkle, SR Ames | 24.63 |
| 11 |  Cassie Rounds, SRDowling Catholic | 24.64 |
| 12 |  Abby Lear, SRMuscatine | 24.65 |
| 13 | Jessi Wigham, JR Waukee | 24.66 |
| 14 | Taylor Judas, SO Waukee | 24.76 |
| 15 | Nora Kemp, SO Waukee | 24.79 |